A fluorescence maikorosikopu imhando yemaikorosikopu yekuona iyo inoshandisa chiedza chepamusoro-soro kuvhenekera mufananidzo uye kufadza fluorochromes mumuenzaniso. Kuvhenekera kwemuenzaniso kunowanzoitwa nechiedza chinobudisa chiedza che ultraviolet. Iwo anoshandiswa zvakanyanya mubiology, zvekurapa uye maindasitiri minda.
Fluorescence microscope inoshanda sei?
A fluorescence microscope inoshandisa mercury kana xenon rambi kugadzira ultraviolet chiedza. Chiedza chinouya mumicroscope uye chinorova girazi re dichroic - girazi rinotaridza rumwe rutivi rwewavelengths uye rinobvumira imwe mutsara kupfuura. Iyo dichroic girazi inoratidzira ultraviolet mwenje kusvika kumuenzaniso. Mimwe mienzaniso fluoresce yakasikwa pasi pe ultraviolet chiedza nokuti ine fluorescent zvinhu zvakadai se chlorophyll. Kana mufananidzo unofanirwa kutariswa usingaite fluoresce, unogona kusvibiswa nedhayi dzefluorescent dzinonzi fluorochromes.
Chii chinonzi fluorescence microscope inosanganisira?
1. Chiedza Chinobva
Iwo anowanzo mwenje masosi ndeye mercury, xenon, uye maLED. Mercury inopa yakanakisa mhando yechiedza kune fluorescence maikorosikopu. Ma LED ari kuwedzera kufarirwa nekuti anodhura zvishoma pane mamwe masosi uye anoshandisa simba shoma.
| Mercury Mwenje | Xenon Lamp | LED | |
| Wavelength yeEmitted Light | 350-370 nm | 400nm ~ 450nm | 400-700 nm |
| Hupenyu hwese(awa) | 200-300 | 400-600 | 10000 |
| Advantage | 1. High Intensity Chiedza, Inonyanya Kuzivikanwa Chiedza Sources. 2. Inobudisa ultraviolet yakasimba uye blue-violet kuti inakidze marudzi ose efluorophores. 3. Mifananidzo Yakajeka uye ine mavara. | 1. The spectral intensity yakagadzikana. 2. Yakasimba spectral intensity mu infrared uye mid-infrared. | 1. Nyore kutsiva. 2. Hapana chikonzero chekudziya. 3. Inogona kubatidzwa nekudzima ipapo ipapo. 4. Kudzora chiedza chinobva kusimba. |
| Kukanganisa | 1. Hupenyu Hupfupi. 2. Nguva refu yekudziya. | 1. Special low voltage DC power box inodiwa pakupisa kwakanyanya. 2. Zvakanyanya Kudhura kupfuura Mercury Lamp | 1. Chiedza cheUV hachina simba pane mercury rambi. 2.Kureba kwechiedza kwega kwega kunoda LED yakaparadzana nekuda kwenzvimbo dzakatetepa dzavanoburitsa. |
Mercury Fluorescent Attachment
LED Fluorescent Attachment
2. Sefa Inofadza
Sefa yekukurudzira yakakosha pakushanda kweiyo fluorescence microscope. Inopfuudza chiedza chehupfupi hwewavelength, iyo dhayi yefluorescent inogona kutora. Zvakare, inovharira mamwe manyuko echiedza chinonakidza.
Sefa yekunakisa(B,G,U,V)
3. Dichroic Mirror
Iyo dichroic girazi imhando ye optical sefa inotaridza mwenje kune mamwe wavelengths ichitumira vamwe. Inoshandiswa mune fluorescence maikorosikopu kuparadzanisa mafambiro uye emission wavelengths.
4. Emission Sefa
Iyo emission sefa inongopfuura mawavelengths anoburitswa nefluorophore uye inovharira mwenje wese usingadiwe kunze kwebhendi iri - kunyanya mafungu eexcitation.
5. Fluorescent dhayi
Madhayi efluorescent maorganic compounds ane pfuma yefluorescence, maanogona kugadzira mufananidzo wefluorescent nekuburitsa mwenje wegirinhi unosiyanisa zvakanyanya mushure mekufadzwa nechiedza chinopenya kwazvo cheultraviolet. Anowanzo shandiswa madhayi efluorescent ndeaya; DAPI (49,6-diamidino-2-phenylindole), acridine orange, auramine-rhodamine, Alexa Fluors, kana DyLight 488.
Mangani emhando dzefluorescence maikorosikopu?
1. Upright Epifluorescence Maikorosikopu:
Ndiyo mhando yakajairika yefluorescence microscope. Kukurudzira kwefluorophore uye kuonekwa kwefluorescence kunoitwa kuburikidza nenzira imwechete yechiedza (kureva kuburikidza nechinangwa). Ruzhinji rwemamaikorosikopu efluorescence, kunyanya ayo anoshandiswa musainzi yehupenyu, ndeepifluorescence dhizaini.
BS-2081F Tsvagiridzo yeFluorescent Biological Maikorosikopu
2. Confocal Fluorescence Maikorosikopu:
Confocal Fluorescence Microscope: Iyi mhando yefluorescence maikorosikopu inosanganisa laser scanning ine fluorescent mwenje kuburitsa mufananidzo. Inogona kushandiswa mumhando dzakasiyana siyana dzekushandisa, sekufunda maseru uye matishu, kuona mapuroteni nezvimwe zvinhu mukati memasero, uye kuyera ukobvu hwezvinhu.
BS-2081F Tsvagiridzo yeFluorescent Biological Maikorosikopu
3. Inverted Fluorescence Microscope
Rudzi urwu rwechiedza chemicroscope uye condenser zviri pamusoro, zvakatarisa pasi. Iko kona yekuvhenekera inofanira kunge iri pa90 degrees maererano nechepamusoro pemuenzaniso uri kuongororwa.
BS-2095F Inverted Fluorescent Maikorosikopu
Ndezvipi zvakanakira uye zvakaipira zvefluorescence microscopes?
Zvakanakira:
- Inogonesa kucherechedzwa kwemasero mune zvipenyu pasina kukuvadza
-Inopa yakakwirira resolution mifananidzo ine chaiyo mavara
- Inobvumira kudzidza kwemaitiro ehupenyu mumaseru
- Inoshandiswa kuona akasiyana marudzi emamorekuru mukati memasero, senge mapuroteni kana nucleic acids.
Zvakaipa:
-Inongobvumira kucherechedzwa kwezvimiro zvakati mukati mesero yakaiswa nedhayi refluorescent.
-The photobleaching nekuda kwe electron excitation panguva ye fluorescence inogona kukanganisa reactive molecules ye fluorescent dyes. Nekuda kweizvozvo, iwo madhayi anoshanda anogona kurasikirwa nemakemikari eiyo fluorescence emission intensity.
-Masero anobatwa nephototoxic effect mushure mekusvibiswa ne fluorescent dyes, sezvo ma molecules efluorophore anotora mafotoni emagetsi kubva kupfupi-wavelength chiedza.
Ndeapi mashandisirwo eiyo fluorescence microscope?
Fluorescence microscopes inoshandiswa zvakanyanya munzvimbo dzakasiyana dzekutsvagisa nekushandisa dzinosanganisira biochemistry, cell biology, microbiology, immunology, uye mushonga.
1. Muchikamu chebiology, fluorescence microscope inogonesa kunyatsozivikanwa uye kwakadzama kwemasero uye submicroscopic cellular components uye mabasa nerubatsiro rwe fluorescent dye labeling.
2. Munzvimbo yezvokurapa, maikorosikopu efluorescence anogona kushandisa fluorescent reagents kuona kuvepo uye kuparadzirwa kwebhakitiriya nemavhairasi, kana kubatsira mukunyora zvinangwa zvekuvhiya kuti kuvhiyiwa kuve nyore.
3. Mumunda we mineralogy, iyo fluorescence microscope inowanzoshandiswa kudzidza zvinhu nezvinongoerekana zvaita fluorescence properties, zvakadai seasphalt, petroleum, marasha, graphene oxide uye mamwe maminerari.
4. Muzvigadzirwa zvesainzi, fluorescence maikorosikopu inogona kushandiswa muindasitiri yemachira kana indasitiri yemapepa kuongorora fiber-based materials.
Nguva yekutumira: Mar-14-2023