Fluorescence microscopy yakasandura kugona kwedu kuona nekudzidza zvipenyu zvipenyu, zvichiita kuti tipinde mukati menyika yakaomarara yemasero nemamorekuru. Chinhu chakakosha chefluorescence microscopy ndiyo sosi yemwenje inoshandiswa kufadza mamorekuru efluorescent mukati memuenzaniso. Kwemakore, magetsi akasiyana-siyana ave achishandiswa, chimwe nechimwe chiine unhu hwakasiyana uye zvakanakira.
1. Mercury Mwenje
Iyo yakakwira-pressure mercury rambi, kubva pa50 kusvika 200 watts, inogadzirwa uchishandisa quartz girazi uye ine denderedzwa muchimiro. Iine humwe huwandu hwemercury mukati. Kana ichishanda, kubuda kunoitika pakati pema electrode maviri, zvichiita kuti mercury ibude, uye kumanikidzwa kwemukati munharaunda kunowedzera nokukurumidza. Izvi zvinowanzotora maminitsi mashanu kusvika gumi nemashanu.
Kuburitswa kwemwenje we-high-pressure mercury kunobva mukuparara uye kuderedzwa kwemamorekuru emekiyuri panguva yekubuda kweelectrode, zvichitungamira mukuburitswa kwemafotoni akajeka.
Inoburitsa yakasimba ultraviolet uye yebhuruu-violet mwenje, ichiita kuti ive yakakodzera kune inonakidza akasiyana efluorescent zvinhu, ndosaka ichishandiswa zvakanyanya mufluorescence microscopy.
2. Xenon Mwenje
Imwe inowanzoshandiswa chena mwenje sosi mune fluorescence microscopy ndeye xenon rambi. Mwenje weXenon, semarambi emercury, anopa huwandu hwakawanda hwewavelengths kubva kuultraviolet kusvika pedyo-infrared. Nekudaro, ivo vanosiyana mune yavo excitation spectra.
Marambi eMercury anonangana nekubuda kwawo munzvimbo dziri pedyo-ultraviolet, bhuruu, uye girinhi, izvo zvinovimbisa kugadzirwa kwemasaini anopenya efluorescent asi anouya neakasimba phototoxicity. Nekuda kweizvozvo, marambi eHBO anowanzo kuchengeterwa masamples akagadziriswa kana isina simba fluorescence imaging. Mukupesana, xenon mwenje masosi ane akatsetseka excitation profile, achibvumira kusimba kuenzanisa pane akasiyana wavelengths. Hunhu uhu hunobatsira pakushandisa senge calcium ion concentration yezviyero. Mwenje weXenon unoratidzawo kunakidzwa kwakasimba mune iri pedyo-infrared renji, kunyanya kutenderedza 800-1000 nm.
XBO marambi ane zvinotevera zvakanaka pamusoro peHBO marambi:
① Kuwedzera yunifomu spectral intensity
② Yakasimba spectral intensity munzvimbo ye infrared uye yepakati-infrared
③ Yakawedzera simba kubuda, zvichiita kuti zvive nyore kusvika kune chinangwa chekuvhura.
3. LEDs
Mumakore achangopfuura, mukwikwidzi mutsva wakabuda munzvimbo yefluorescence microscopy light sources: LEDs. Ma LED anopa mukana wekukurumidza-ku-off switching mumamilliseconds, kuderedza nguva yekuratidzwa uye kuwedzera hupenyu hwemasampuli akapfava. Uyezve, mwenje we LED unoratidza kuora nekukurumidza uye kwakaringana, zvichideredza zvakanyanya phototoxicity panguva yekuedza kwesero kwenguva refu.
Kuenzaniswa nemwenje machena masosi, maLED anowanzo buda mukati meyakamanikana excitation spectrum. Nekudaro, akawanda mabhendi e-LED aripo, achibvumira kune akasiyana-siyana-mavara e-fluorescence maapplication, achiita ma LEDs ive iri kuwedzera mukurumbira sarudzo mune yemazuva ano fluorescence microscopy setups.
4. Lasers Chiedza Chitubu
Laser light sources akanyanya monochromatic uye anotungamira, achivaita akanakira yakakwira-resolution microscope, kusanganisira super-resolution matekiniki akadai seSTED (Stimulated Emission Depletion) uye PALM (Photoactivated Localization Microscopy). Chiedza cheLaser chinowanzo sarudzwa kuti chienderane nechaiyo excitation wavelength inodiwa kune chinangwa chefluorophore, ichipa yakanyanya kusarudzwa uye nemazvo mukunakidzwa kwefluorescence.
Sarudzo yechiedza chefluorescence microscope chiedza chinoenderana nezvinodiwa zvekuedza uye maitiro emuenzaniso. Ndapota inzwa wakasununguka kutibata nesu kana uchida chero rubatsiro
Nguva yekutumira: Sep-13-2023